@PhdThesis{diss_mods_00000542, author = {Grobe, Olaf}, title = {Funktionelle Charakterisierung des p53-Zielgens IEX-1 durch ribozymvermittelte Inhibition der Genexpression}, year = {2002}, publisher = {Christian-Albrechts-Universit{\"a}t zu Kiel}, address = {Kiel}, keywords = {Hammerhead-Ribozyme; Zellzyklus; Apoptose; pBabePuro}, abstract = {The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis.}, url = {https://macau.uni-kiel.de/receive/diss_mods_00000542}, file = {:https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00000542/d542.pdf:PDF}, language = {de} }