000K  utf8
1100  $c2002
1500  ger
2050  urn:nbn:de:gbv:8-diss-5423
3000  Grobe, Olaf
4000  Funktionelle Charakterisierung des p53-Zielgens IEX-1 durch ribozymvermittelte Inhibition der Genexpression$hChristian-Albrechts-Universität zu Kiel  [Grobe, Olaf]
4030  Kiel$nChristian-Albrechts-Universität zu Kiel
4209  The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis.
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-5423$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/diss_mods_00000542
5051  610
5550  Apoptose
5550  Hammerhead-Ribozyme
5550  pBabePuro
5550  Zellzyklus