000K utf8 1100 $c2002 1500 ger 2050 urn:nbn:de:gbv:8-diss-5423 3000 Grobe, Olaf 4000 Funktionelle Charakterisierung des p53-Zielgens IEX-1 durch ribozymvermittelte Inhibition der Genexpression$hChristian-Albrechts-Universität zu Kiel [Grobe, Olaf] 4030 Kiel$nChristian-Albrechts-Universität zu Kiel 4209 The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis. 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-5423$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/diss_mods_00000542 5051 610 5550 Apoptose 5550 Hammerhead-Ribozyme 5550 pBabePuro 5550 Zellzyklus