@PhdThesis{diss_mods_00026213,
  author = 	{Huang, Xiufeng},
  title = 	{Short Tandem Repeat Profiles In Ovarian Carcinoma Cells During Primary Culture},
  year = 	{2019},
  publisher = 	{Christian-Albrechts-Universit{\"a}t zu Kiel},
  address = 	{Kiel},
  keywords = 	{Ovarian Cancer; Short Tandem Repeat; Primary Culture; Authentication; STR profiling; Genetic fingerprint},
  abstract = 	{Purpose Cell authentication is a necessary procedure to
avoid scientific data from cell culture experiments with
cross-contamination or false classification. A genetic fingerprint
pattern of a specimen by short tandem repeats
(STR) is self-evident. Due to high amount of chromosomal
rearrangements, known in epithelia ovary cancer cells and
the instable STR pattern described in other tumour entities
like leukaemia, this study explores the suitability of STR
profiling for primary cultured epithelial ovary cancer cells.
Methods STR profiles of epithelial ovary cancers of 16
patients were compared with corresponding blood and
corresponding primary cell cultures. The primary cell
cultures of epithelial ovary tumours were passaged up to 28
times. In between, cultures were cryo conserved and
recultured again, two to five times per patient.
Results In two cases, the STR pattern of tumour lost
alleles (1/16 and 3/16) in comparison of corresponding
STR-pattern from blood. In comparison to blood, cell
culture of a third case, lost four alleles (4/16) accompanied
with morphologic changes after 14th passage. It is equal
after cryo conservation of the seventh passage from the
same patient. The only changes in STR profiles we recognized
are losses of alleles. Remaining STR markers
allow authentication.
Conclusions Very likely, the allelic drop-outs beyond
passage 14 assume complex genetic losses of heterozygosis
resulting in changed growth behaviour of cells. All other
STR-profiles of remaining 15 patients analysed in this
study are stable over all passages and freeze--thaw processes.
Thus, ovary cancer cell cultures in research should
be authenticated by STR-profile in general.},
  url = 	{https://macau.uni-kiel.de/receive/diss_mods_00026213},
  file = 	{:https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00008513/short_tandem_repeats_in_ovarian_cancer_during_primary_culture-2019-10-21-xiufeng_huang.pdf:PDF},
  language = 	{en}
}