000K utf8 1100 $c2019 1500 eng 2050 urn:nbn:de:gbv:8-diss-262133 3000 Huang, Xiufeng 4000 Short Tandem Repeat Profiles In Ovarian Carcinoma Cells During Primary Culture$hChristian-Albrechts-Universität zu Kiel [Huang, Xiufeng] 4030 Kiel$nChristian-Albrechts-Universität zu Kiel 4209 Purpose Cell authentication is a necessary procedure to avoid scientific data from cell culture experiments with cross-contamination or false classification. A genetic fingerprint pattern of a specimen by short tandem repeats (STR) is self-evident. Due to high amount of chromosomal rearrangements, known in epithelia ovary cancer cells and the instable STR pattern described in other tumour entities like leukaemia, this study explores the suitability of STR profiling for primary cultured epithelial ovary cancer cells. Methods STR profiles of epithelial ovary cancers of 16 patients were compared with corresponding blood and corresponding primary cell cultures. The primary cell cultures of epithelial ovary tumours were passaged up to 28 times. In between, cultures were cryo conserved and recultured again, two to five times per patient. Results In two cases, the STR pattern of tumour lost alleles (1/16 and 3/16) in comparison of corresponding STR-pattern from blood. In comparison to blood, cell culture of a third case, lost four alleles (4/16) accompanied with morphologic changes after 14th passage. It is equal after cryo conservation of the seventh passage from the same patient. The only changes in STR profiles we recognized are losses of alleles. Remaining STR markers allow authentication. Conclusions Very likely, the allelic drop-outs beyond passage 14 assume complex genetic losses of heterozygosis resulting in changed growth behaviour of cells. All other STR-profiles of remaining 15 patients analysed in this study are stable over all passages and freeze–thaw processes. Thus, ovary cancer cell cultures in research should be authenticated by STR-profile in general. 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-262133$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/diss_mods_00026213 5051 610 5550 Authentication 5550 Genetic fingerprint 5550 Ovarian Cancer 5550 Primary Culture 5550 Short Tandem Repeat 5550 STR profiling