000K utf8 1100 $c2019 1500 eng 2050 urn:nbn:de:gbv:8-mods-2019-00009-0 3000 Makowka, Alexander 4000 Glycolytic shunts are anaplerotic reactions for the Calvin-Benson-Bassham cycle$hChristian-Albrechts-Universität zu Kiel [Makowka, Alexander] 4000 Glykolytische Shunts sind anaplerotische Reaktionen für den Calvin-Benson-Bassham Zyklus$hChristian-Albrechts-Universität zu Kiel [Makowka, Alexander] 4030 Kiel$nChristian-Albrechts-Universität zu Kiel 4209 In this work, it was shown that the cyanobacterium Synechocystis employs three glycolytic shunts as anaplerotic reactions for the CBB cycle. The three shunts, named 'PGI shunt', 'OPP shunt' and 'ED shunt', not only mediated anaplerosis of glucose that was taken up under mixotrophic conditions but also of glycogen-derived glucose under photoautotrophic conditions. It could be shown that degradation of glycogen was able to improve restarting of the CBB cycle after a period of darkness. This suggests, that the cellular glycogen pool in cyanobacteria functions as a metabolic buffer that can be tapped upon demand in order to improve flux in the CBB cycle. The three glycolytic shunts thereby function as anaplerotic reactions. It can be assumed that glycolytic shunts not only help to control metabolite stoichiometry in the CBB cycle, but also to influence the cellular ATP and NADPH pools and thus provide a tool to improve synchronization with the photosynthetic light reaction. 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8-mods-2019-00009-0$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00000018 5051 580 5550 6803 5550 anaplerotic 5550 autocatalytic 5550 Benson 5550 Calvin 5550 Calvin-Benson 5550 Calvin-Benson-Bassham 5550 CBB 5550 cyanobacteria 5550 cycle 5550 ED 5550 Entner 5550 Entner-Doudoroff 5550 OPP 5550 PGI 5550 Synechocystis