000K  utf8
1100  $c2019
1500  eng
2050  urn:nbn:de:gbv:8-mods-2019-00126-9
2051  10.3389/fmicb.2019.01793
3000  Yang, Wentao
3010  Dierking, Katja
3010  Dirksen, Philipp
3010  Graf von der Schulenburg, Hinrich
3010  Kaleta, Christoph
3010  Leippe, Matthias
3010  Pees, Barbara
3010  Petersen, Carola
3010  Rosenstiel, Philip
3010  Tholey, Andreas
3010  Waschina, Silvio
3010  Zimmermann, Johannes
4000  The Inducible Response of the Nematode Caenorhabditis elegans to Members of Its Natural Microbiota Across Development and Adult Life  [Yang, Wentao]
4209  The biology of all organisms is influenced by the associated community of microorganisms. In spite of its importance, it is usually not well understood how exactly this microbiota affects host functions and what are the underlying molecular processes. To rectify this knowledge gap, we took advantage of the nematode Caenorhabditis elegans as a tractable, experimental model system and assessed the inducible transcriptome response after colonization with members of its native microbiota. For this study, we focused on two isolates of the genus Ochrobactrum. These bacteria are known to be abundant in the nematode's microbiota and are capable of colonizing and persisting in the nematode gut, even under stressful conditions. The transcriptome response was assessed across development and three time points of adult life, using general and C. elegans-specific enrichment analyses to identify affected functions. Our assessment revealed an influence of the microbiota members on the nematode's dietary response, development, fertility, immunity, and energy metabolism. This response is mainly regulated by a GATA transcription factor, most likely ELT-2, as indicated by the enrichment of (i) the GATA motif in the promoter regions of inducible genes and (ii) of ELT-2 targets among the differentially expressed genes. We compared our transcriptome results with a corresponding previously characterized proteome data set, highlighting a significant overlap in the differentially expressed genes, the affected functions, and ELT-2 target genes. Our analysis further identified a core set of 86 genes that consistently responded to the microbiota members across development and adult life, including several C-type lectin-like genes and genes known to be involved in energy metabolism or fertility. We additionally assessed the consequences of induced gene expression with the help of metabolic network model analysis, using a previously established metabolic network for C. elegans. This analysis complemented the enrichment analyses by revealing an influence of the Ochrobactrum isolates on C. elegans energy metabolism and furthermore metabolism of specific amino acids, fatty acids, and also folate biosynthesis. Our findings highlight the multifaceted impact of naturally colonizing microbiota isolates on C. elegans life history and thereby provide a framework for further analysis of microbiota-mediated host functions.
4950  https://doi.org/10.3389/fmicb.2019.01793$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8-mods-2019-00126-9$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00000079
5051  610
5550  Caenorhabditis elegans
5550  Metabolic Network Model
5550  Microbiota
5550  Ochrobactrum
5550  Rna-seq
5550  Transcriptomics