000K  utf8
1100  $c2020
1500  eng
2050  urn:nbn:de:gbv:8-mods-2020-00457-0
2051  10.3389/fimmu.2020.00661
3000  Parackova, Zuzana
3010  Hasler, Robert
3010  Klocperk, Adam
3010  Petruzelkova, Lenka
3010  Pruhova, Stepanka
3010  Sediva, Anna
3010  Sumnik, Zdenek
3010  Vrabcova, Petra
3010  Zentsova, Irena
4000  Neutrophil Extracellular Trap Induced Dendritic Cell Activation Leads to Th1 Polarization in Type 1 Diabetes  [Parackova, Zuzana]
4209  Neutrophils releasing neutrophil extracellular traps (NETs) infiltrate the pancreas prior to type 1 diabetes (T1D) onset; however, the precise nature of their contribution to disease remains poorly defined. To examine how NETs affect immune functions in T1D, we investigated NET composition and their effect on dendritic cells (DCs) and T lymphocytes in T1D children. We showed that T1D patient NET composition differs substantially from that of healthy donors and that the presence of T1D-NETs in a mixed peripheral blood mononuclear cell culture caused a strong shift toward IFNγ-producing T lymphocytes, mediated through activation of innate immunity cells in T1D samples. Importantly, in a monocyte-derived DC (moDC) culture, NETs induced cytokine production, phenotypic change and IFNγ-producing T cells only in samples from T1D patients but not in those from healthy donors. RNA-seq analysis revealed that T1D-NETs presence causes TGFβ downregulation and IFNα upregulation and creates pro-T1D signature in healthy moDCs.
4950  https://doi.org/10.3389/fimmu.2020.00661$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8-mods-2020-00457-0$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00000858
5051  610
5550  Autoimmunity
5550  innate immunity
5550  Monocyte-derived Dendritic Cells
5550  Net
5550  Netosis
5550  Neutrophils
5550  type 1 diabetes