000K  utf8
1100  $c2020
1500  eng
2050  urn:nbn:de:gbv:8:3-2021-00036-5
2051  10.3390/md19010006
3000  Utermann, Caroline
3010  Blümel, Martina
3010  Echelmeyer, Vivien A
3010  Oppong-Danquah, Ernest
3010  Tasdemir, Deniz
4000  Diversity, Bioactivity Profiling and Untargeted Metabolomics of the Cultivable Gut Microbiota of Ciona intestinalis  [Utermann, Caroline]
4209  It is widely accepted that the commensal gut microbiota contributes to the health and well-being of its host. The solitary tunicate Ciona intestinalis emerges as a model organism for studying host-microbe interactions taking place in the gut, however, the potential of its gut-associated microbiota for marine biodiscovery remains unexploited. In this study, we set out to investigate the diversity, chemical space, and pharmacological potential of the gut-associated microbiota of C. intestinalis collected from the Baltic and North Seas. In a culture-based approach, we isolated 61 bacterial and 40 fungal strains affiliated to 33 different microbial genera, indicating a rich and diverse gut microbiota dominated by Gammaproteobacteria. In vitro screening of the crude microbial extracts indicated their antibacterial (64% of extracts), anticancer (22%), and/or antifungal (11%) potential. Nine microbial crude extracts were prioritized for in-depth metabolome mining by a bioactivity- and chemical diversity-based selection procedure. UPLC-MS/MS-based metabolomics combining automated (feature-based molecular networking and in silico dereplication) and manual approaches significantly improved the annotation rates. A high chemical diversity was detected where peptides and polyketides were the predominant classes. Many compounds remained unknown, including two putatively novel lipopeptides produced by a Trichoderma sp. strain. This is the first study assessing the chemical and pharmacological profile of the cultivable gut microbiota of C. intestinalis.
4950  https://doi.org/10.3390/md19010006$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2021-00036-5$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00000958
5051  333.7
5550  Anticancer activity
5550  Antimicrobial activity
5550  Ciona intestinalis
5550  Feature-based Molecular Networking
5550  Gut-associated Microbiota
5550  In Silico Ms/ms-based Dereplication
5550  marine natural products
5550  Tunicate
5550  Untargeted Metabolomics