000K  utf8
1100  $c2021
1500  eng
2050  urn:nbn:de:gbv:8:3-2021-00462-0
2051  10.3390/v13050801
3000  Anastasiou, Olympia E
3010  Eis-Hübinger, Anna Maria
3010  Holtkamp, Caroline
3010  Krumbholz, Andi
3010  Schäfer, Miriam
3010  Schön, Frieda
4000  Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test  [Anastasiou, Olympia E]
4209  The availability of simple SARS-CoV-2 detection methods is crucial to contain the COVID-19 pandemic. This study examined whether a commercial LAMP assay can reliably detect SARS-CoV-2 genomes directly in respiratory samples without having to extract nucleic acids (NA) beforehand. Nasopharyngeal swabs (NPS, n = 220) were tested by real-time reverse transcription (RT)-PCR and with the LAMP assay. For RT-PCR, NA were investigated. For LAMP, NA from 26 NPS in viral transport medium (VTM) were tested. The other 194 NPS were analyzed directly without prior NA extraction (140 samples in VTM; 54 dry swab samples stirred in phosphate buffered saline). Ten NPS were tested directly by LAMP using a sous-vide cooking unit. The isothermal assay demonstrated excellent specificity (100%) but moderate sensitivity (68.8%), with a positive predictive value of 1 and a negative predictive value of 0.65 for direct testing of NPS in VTM. The use of dry swabs, even without NA extraction, improved the analytical sensitivity; up to 6% of samples showed signs of inhibition. LAMP could be performed successfully with a sous-vide cooking unit. This technique is very fast, requires little laboratory resources, and can replace rapid antigen tests or verify reactive rapid tests on-site.
4950  https://doi.org/10.3390/v13050801$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2021-00462-0$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00001505
5051  610
5550  Covid-19
5550  COVID-19
5550  COVID-19 Nucleic Acid Testing
5550  Direct Testing
5550  DNA, Viral
5550  Humans
5550  LAMP
5550  Loop-mediated Isothermal Amplification
5550  Molecular Diagnostic Techniques
5550  Nasopharynx
5550  Nucleic Acid Amplification Techniques
5550  Nucleic acids
5550  RT-PCR
5550  Sars-cov-2
5550  SARS-CoV-2
5550  Sensitivity and Specificity
5550  Specimen Handling