000K utf8 1100 $c2021 1500 eng 2050 urn:nbn:de:gbv:8:3-2021-00785-0 2051 10.3390/md19100542 3000 Eckert, Thomas 3010 Burg-Roderfeld, Monika 3010 Engelhard, Peter 3010 Hesse, Karsten 3010 Hudson, John W. 3010 Jährling-Butkus, Mahena 3010 Kožár, Tibor 3010 Kozarova, Anna 3010 Louton, Helen 3010 Petridis, Athanasios K. 3010 Schauer, Roland 3010 Siebert, Hans-Christian 3010 Steinmeyer, Jürgen 3010 Zhang, Ning 3010 Zhang, Ruiyan 4000 Efficacy of Chondroprotective Food Supplements Based on Collagen Hydrolysate and Compounds Isolated from Marine Organisms † [Eckert, Thomas] 4209 Osteoarthritis belongs to the most common joint diseases in humans and animals and shows increased incidence in older patients. The bioactivities of collagen hydrolysates, sulfated glucosamine and a special fatty acid enriched dog-food were tested in a dog patient study of 52 dogs as potential therapeutic treatment options in early osteoarthritis. Biophysical, biochemical, cell biological and molecular modeling methods support that these well-defined substances may act as effective nutraceuticals. Importantly, the applied collagen hydrolysates as well as sulfated glucosamine residues from marine organisms were strongly supported by both an animal model and molecular modeling of intermolecular interactions. Molecular modeling of predicted interaction dynamics was evaluated for the receptor proteins MMP-3 and ADAMTS-5. These proteins play a prominent role in the maintenance of cartilage health as well as innate and adapted immunity. Nutraceutical data were generated in a veterinary clinical study focusing on mobility and agility. Specifically, key clinical parameter (MMP-3 and TIMP-1) were obtained from blood probes of German shepherd dogs with early osteoarthritis symptoms fed with collagen hydrolysates. Collagen hydrolysate, a chondroprotective food supplement was examined by high resolution NMR experiments. Molecular modeling simulations were used to further characterize the interaction potency of collagen fragments and glucosamines with protein receptor structures. Potential beneficial effects of collagen hydrolysates, sulfated glycans (i.e., sulfated glucosamine from crabs and mussels) and lipids, especially, eicosapentaenoic acid (extracted from fish oil) on biochemical and physiological processes are discussed here in the context of human and veterinary medicine. 4950 https://doi.org/10.3390/md19100542$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2021-00785-0$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00002134 5051 570 5550 Adamts-5 5550 Collagen Hydrolysate 5550 Eicosapentaenoic Acid (Epa) 5550 Mmp-3 5550 Osteoarthritis 5550 Sialic acids 5550 Sulfated N-Acetyl Glucosamine