000K utf8 1100 $c2021 1500 eng 2050 urn:nbn:de:gbv:8:3-2022-00173-9 2051 10.3390/app11093901 3000 Satankar, Varsha 3010 Changan, Sushil 3010 Jhodkar, Durwesh 3010 Kumar, Manoj 3010 Mageshwaran, Vellaichamy 3010 Mekhemar, Mohamed 3010 Singh, Mohan 4000 Cottonseed Kernel Powder as a Natural Health Supplement$dAn Approach to Reduce the Gossypol Content and Maximize the Nutritional Benefits [Satankar, Varsha] 4209 Cottonseed is one of the important by-products of the cotton crop. Researchers claim that cottonseed with less than 0.45% of gossypol is quite good for human consumption and animal feeding because it is a rich source of protein, edible oil, and energy. Total and free gossypols are the influencing parameters that reduce the edible nature of the cottonseed. In the present work, multiple quadratic regression models have been prepared to predict the reduction in the free and total gossypol percent. This response surface method (RSM)-based approach was applied to investigate the combined effect between input parameters such as acetone level, time of extraction, liquid-to-solid ratio (LSR), and the number of extraction cycles, whereas output responses are free and total gossypol reduction percentage. Analysis of Variance (ANOVA) has been performed to determine the highly significant parameter. The optimum combination of input parameters was determined using the RSM-based desirability approach, and confirmatory experiments were performed to validate the combination. Results revealed that the number of extraction cycles and liquid-to-solid ratio significantly affects the reduction of free and total gossypol levels. The values of r-square were found above 0.9, which indicates that the developed models are suitable and reliable for predicting free and total gossypol reduction percentage. 4950 https://doi.org/10.3390/app11093901$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00173-9$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00002614 5051 600 5051 610 5550 cottonseed 5550 gossypol 5550 optimization 5550 response surface methodology 5550 toxicity