000K  utf8
1100  $c2021
1500  eng
2050  urn:nbn:de:gbv:8:3-2022-00221-8
2051  10.3390/ijms22083864
3000  Heneweer, Carola
3010  Kalthoff, Holger
3010  Kolesnick, Richard
3010  Larson, Steven
3010  Peñate Medina, Oula Antti Rafael
3010  Peñate Medina, Tuula
3010  Tower, Robert
4000  Acid-Sphingomyelinase Triggered Fluorescently Labeled Sphingomyelin Containing Liposomes in Tumor Diagnosis after Radiation-Induced Stress  [Heneweer, Carola]
4209  In liposomal delivery, a big question is how to release the loaded material into the correct place. Here, we will test the targeting and release abilities of our sphingomyelin-consisting liposome. A change in release parameters can be observed when sphingomyelin-containing liposome is treated with sphingomyelinase enzyme. Sphingomyelinase is known to be endogenously released from the different cells in stress situations. We assume the effective enzyme treatment will weaken the liposome making it also leakier. To test the release abilities of the SM-liposome, we developed several fluorescence-based experiments. In in vitro studies, we used molecular quenching to study the sphingomyelinase enzyme-based release from the liposomes. We could show that the enzyme treatment releases loaded fluorescent markers from sphingomyelin-containing liposomes. Moreover, the release correlated with used enzymatic activities. We studied whether the stress-related enzyme expression is increased if the cells are treated with radiation as a stress inducer. It appeared that the radiation caused increased enzymatic activity. We studied our liposomes' biodistribution in the animal tumor model when the tumor was under radiation stress. Increased targeting of the fluorescent marker loaded to our liposomes could be found on the site of cancer. The liposomal targeting in vivo could be improved by radiation. Based on our studies, we propose sphingomyelin-containing liposomes can be used as a controlled release system sensitive to cell stress.
4950  https://doi.org/10.3390/ijms22083864$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00221-8$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00002624
5051  610
5550  Animals
5550  Catalysis
5550  Cell Line, Tumor
5550  Cell Stress
5550  Controlled release
5550  Disease Models, Animal
5550  Drug Delivery
5550  Enzyme Activation
5550  Fluorescence
5550  Fluorescent Dyes
5550  Liposome
5550  Liposomes
5550  Mice
5550  Molecular Imaging
5550  Neoplasms
5550  Optical Imaging
5550  Quenching
5550  Sphingomyelinase
5550  Sphingomyelin Phosphodiesterase
5550  Sphingomyelins
5550  Staining and Labeling
5550  Stress, Physiological