000K  utf8
1100  $c2021
1500  eng
2050  urn:nbn:de:gbv:8:3-2022-00250-8
2051  10.3390/ijms221910536
3000  Bayer, Andreas
3010  Behrendt, Peter
3010  Gläser, Regine
3010  Harder, Jürgen
3010  Kubo, Yusuke
3010  Möbus, Lena
3010  Preuß, Mark
3010  Rademacher, Franziska
3010  Rodewald, Meno
3010  Singh, Michael
3010  Tohidnezhad, Mersedeh
3010  Weitkamp, Jan-Tobias
3010  Wijaya, Bernard
4000  Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts  [Bayer, Andreas]
4209  Platelet concentrate products are increasingly used in many medical disciplines due to their regenerative properties. As they contain a variety of chemokines, cytokines, and growth factors, they are used to support the healing of chronic or complicated wounds. To date, underlying cellular mechanisms have been insufficiently investigated. Therefore, we analyzed the influence of Platelet-Released Growth Factors (PRGF) on human dermal fibroblasts. Whole transcriptome sequencing and gene ontology (GO) enrichment analysis of PRGF-treated fibroblasts revealed an induction of several genes involved in the formation of the extracellular matrix (ECM). Real-time PCR analyses of PRGF-treated fibroblasts and skin explants confirmed the induction of ECM-related genes, in particular transforming growth factor beta-induced protein (TGFBI), fibronectin 1 (FN1), matrix metalloproteinase-9 (MMP-9), transglutaminase 2 (TGM2), fermitin family member 1 (FERMT1), collagen type I alpha 1 (COL1A1), a disintegrin and metalloproteinase 19 (ADAM19), serpin family E member 1 (SERPINE1) and lysyl oxidase-like 3 (LOXL3). The induction of these genes was time-dependent and in part influenced by the epidermal growth factor receptor (EGFR). Moreover, PRGF induced migration and proliferation of the fibroblasts. Taken together, the observed effects of PRGF on human fibroblasts may contribute to the underlying mechanisms that support the beneficial wound-healing effects of thrombocyte concentrate products.
4950  https://doi.org/10.3390/ijms221910536$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00250-8$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00002671
5051  610
5550  Blood Platelets
5550  Cell Movement
5550  Cell Proliferation
5550  Cells, Cultured
5550  Collagen Type I, alpha 1 Chain
5550  Extracellular Matrix
5550  Extracellular matrix (ECM)
5550  Extracellular Matrix Proteins
5550  Fibroblasts
5550  Fibroblasts
5550  Humans
5550  Intercellular Signaling Peptides and Proteins
5550  Platelet-released Growth Factors (Prgf)
5550  Wound healing