000K utf8 1100 $c2021 1500 eng 2050 urn:nbn:de:gbv:8:3-2022-00250-8 2051 10.3390/ijms221910536 3000 Bayer, Andreas 3010 Behrendt, Peter 3010 Gläser, Regine 3010 Harder, Jürgen 3010 Kubo, Yusuke 3010 Möbus, Lena 3010 Preuß, Mark 3010 Rademacher, Franziska 3010 Rodewald, Meno 3010 Singh, Michael 3010 Tohidnezhad, Mersedeh 3010 Weitkamp, Jan-Tobias 3010 Wijaya, Bernard 4000 Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts [Bayer, Andreas] 4209 Platelet concentrate products are increasingly used in many medical disciplines due to their regenerative properties. As they contain a variety of chemokines, cytokines, and growth factors, they are used to support the healing of chronic or complicated wounds. To date, underlying cellular mechanisms have been insufficiently investigated. Therefore, we analyzed the influence of Platelet-Released Growth Factors (PRGF) on human dermal fibroblasts. Whole transcriptome sequencing and gene ontology (GO) enrichment analysis of PRGF-treated fibroblasts revealed an induction of several genes involved in the formation of the extracellular matrix (ECM). Real-time PCR analyses of PRGF-treated fibroblasts and skin explants confirmed the induction of ECM-related genes, in particular transforming growth factor beta-induced protein (TGFBI), fibronectin 1 (FN1), matrix metalloproteinase-9 (MMP-9), transglutaminase 2 (TGM2), fermitin family member 1 (FERMT1), collagen type I alpha 1 (COL1A1), a disintegrin and metalloproteinase 19 (ADAM19), serpin family E member 1 (SERPINE1) and lysyl oxidase-like 3 (LOXL3). The induction of these genes was time-dependent and in part influenced by the epidermal growth factor receptor (EGFR). Moreover, PRGF induced migration and proliferation of the fibroblasts. Taken together, the observed effects of PRGF on human fibroblasts may contribute to the underlying mechanisms that support the beneficial wound-healing effects of thrombocyte concentrate products. 4950 https://doi.org/10.3390/ijms221910536$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00250-8$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00002671 5051 610 5550 Blood Platelets 5550 Cell Movement 5550 Cell Proliferation 5550 Cells, Cultured 5550 Collagen Type I, alpha 1 Chain 5550 Extracellular Matrix 5550 Extracellular matrix (ECM) 5550 Extracellular Matrix Proteins 5550 Fibroblasts 5550 Fibroblasts 5550 Humans 5550 Intercellular Signaling Peptides and Proteins 5550 Platelet-released Growth Factors (Prgf) 5550 Wound healing