000K  utf8
1100  $c2022
1500  eng
2050  urn:nbn:de:gbv:8:3-2022-00559-6
2051  10.3389/fmicb.2022.869666
3000  Bathia, Jay Rajesh
3010  Bosch, Thomas
3010  Fraune, Sebastian
3010  Lachnit, Tim
3010  Rosenstiel, Philip
3010  Schröder, Katja
4000  Symbiotic Algae of Hydra viridissima Play a Key Role in Maintaining Homeostatic Bacterial Colonization  [Bathia, Jay Rajesh]
4209  The freshwater polyp Hydra viridissima (H. viridissima) harbors endosymbiotic Chlorella algae in addition to a species-specific microbiome. The molecular basis of the symbiosis between Hydra and Chlorella has been characterized to be metabolic in nature. Here, we studied the interaction between the extracellularly located microbiota and the algal photobiont, which resides in Hydra's endodermal epithelium, with main focus on Legionella bacterium. We aimed at evaluating the influence of the symbiotic algae on microbial colonization and in shaping the host microbiome. We report that the microbiome composition of symbiotic and aposymbiotic (algae free) H. viridissima is significantly different and dominated by Legionella spp. Hvir in aposymbiotic animals. Co-cultivation of these animals resulted in horizontal transmission of Legionella spp. Hvir bacteria from aposymbiotic to symbiotic animals. Acquisition of this bacterium increased the release of algae into ambient water. From there, algae could subsequently be taken up again by the aposymbiotic animals. The presence of algal symbionts had negative impact on Legionella spp. Hvir and resulted in a decrease of the relative abundance of this bacterium. Prolonged co-cultivation ultimately resulted in the disappearance of the Legionella spp. Hvir bacterium from the Hydra tissue. Our observations suggest an important role of the photobiont in controlling an invasive species in a metacommunity and, thereby, shaping the microbiome.
4950  https://doi.org/10.3389/fmicb.2022.869666$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00559-6$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00002976
5051  610
5550  Co-cultivation
5550  horizontal transmission
5550  Microbiome
5550  Symbiosis
5550  Tripartite Interactions