000K  utf8
1100  $c2017
1500  eng
2050  urn:nbn:de:gbv:8:3-2022-00604-4
2051  10.3390/ijms18071468
3000  Adamski, Vivian
3010  Hattermann-Koch, Kirsten
3010  Held-Feindt, Janka
3010  Lucius, Ralph-Erich
3010  Mentlein, Rolf
3010  Synowitz, Michael
4000  The Chemokine Receptor CXCR6 Evokes Reverse Signaling via the Transmembrane Chemokine CXCL16  [Adamski, Vivian]
4209  Reverse signaling is a signaling mechanism where transmembrane or membrane-bound ligands transduce signals and exert biological effects upon binding of their specific receptors, enabling a bidirectional signaling between ligand and receptor-expressing cells. In this study, we address the question of whether the transmembrane chemokine (C-X-C motif) ligand 16, CXCL16 is able to transduce reverse signaling and investigate the biological consequences. For this, we used human glioblastoma cell lines and a melanoma cell line as in vitro models to show that stimulation with recombinant C-X-C chemokine receptor 6 (CXCR6) or CXCR6-containing membrane preparations induces intracellular (reverse) signaling. Specificity was verified by RNAi experiments and by transfection with expression vectors for the intact CXCL16 and an intracellularly-truncated form of CXCL16. We showed that reverse signaling via CXCL16 promotes migration in CXCL16-expressing melanoma and glioblastoma cells, but does not affect proliferation or protection from chemically-induced apoptosis. Additionally, fast migrating cells isolated from freshly surgically-resected gliomas show a differential expression pattern for CXCL16 in comparison to slowly-migrating cells, enabling a possible functional role of the reverse signaling of the CXCL16/CXCR6 pair in human brain tumor progression in vivo.
4950  https://doi.org/10.3390/ijms18071468$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00604-4$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00003034
5051  610
5550  Cell Communication
5550  Cell Movement
5550  Chemokine CXCL16
5550  Glioma
5550  Humans
5550  Mitogen-Activated Protein Kinase 1
5550  Mitogen-Activated Protein Kinase 3
5550  Phosphorylation
5550  Receptors, Chemokine
5550  Receptors, CXCR6
5550  Signal Transduction