000K utf8 1100 $c2018 1500 eng 2050 urn:nbn:de:gbv:8:3-2022-00619-0 2051 10.3390/ijms19010223 3000 Staats, Stefanie 3010 Kowalewski, Bianca 3010 Kulling, Sabine E 3010 Rieck, Florian T 3010 Rimbach, Gerald 3010 Soukup, Sebastian T 3010 Wagner, Anika Eva 4000 Dietary Resveratrol Does Not Affect Life Span, Body Composition, Stress Response, and Longevity-Related Gene Expression in Drosophila melanogaster [Staats, Stefanie] 4209 In this study, we tested the effect of the stilbene resveratrol on life span, body composition, locomotor activity, stress response, and the expression of genes encoding proteins centrally involved in ageing pathways in the model organism Drosophila melanogaster. Male and female w1118 D. melanogaster were fed diets based on sucrose, corn meal, and yeast. Flies either received a control diet or a diet supplemented with 500 µmol/L resveratrol. Dietary resveratrol did not affect mean, median, and maximal life span of male and female flies. Furthermore, body composition remained largely unchanged following the resveratrol supplementation. Locomotor activity, as determined by the climbing index, was not significantly different between control and resveratrol-supplemented flies. Resveratrol-fed flies did not exhibit an improved stress response towards hydrogen peroxide as compared to controls. Resveratrol did not change mRNA steady levels of antioxidant (catalase, glutathione-S-transferase, NADH dehydrogenase, glutathione peroxidase, superoxide dismutase 2) and longevity-related genes, including sirtuin 2, spargel, and I'm Not Dead Yet. Collectively, present data suggest that resveratrol does not affect life span, body composition, locomotor activity, stress response, and longevity-associated gene expression in w1118 D. melanogaster. 4950 https://doi.org/10.3390/ijms19010223$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00619-0$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00003044 5051 333.7 5051 610 5550 Animals 5550 Antioxidants 5550 Body Composition 5550 Dietary Supplements 5550 Drosophila melanogaster 5550 Drosophila Proteins 5550 Female 5550 Locomotion 5550 Longevity 5550 Male 5550 Oxidative Stress 5550 Resveratrol 5550 Stilbenes