000K utf8 1100 $c2022 1500 eng 2050 urn:nbn:de:gbv:8:3-2022-00834-9 3000 Ohmes, Julia Maria 4000 Fucoidan in Bone Tissue Regeneration$dFrom Cell Type Specific Bioactivities to the Development of a Hydrogel-based Delivery System$hChristian-Albrechts-Universität zu Kiel [Ohmes, Julia Maria] 4000 Fucoidan in der Knochenregeneration$dVon Zellspezifischen Bioaktivitäten zur Entwicklung eines Hydrogelbasierten Trägersystems$hChristian-Albrechts-Universität zu Kiel [Ohmes, Julia Maria] 4030 Kiel$nChristian-Albrechts-Universität zu Kiel 4209 Fucoidans are sulfated polysaccharides found in the cell wall of brown algae. Due to their ample effects on angiogenesis, osteogenesis and inflammation, these polysaccharides are promising bioactive compounds for the application in regenerative medicine. However, the chemical structure of fucoidan which determines the bioactivities is dependent on many parameters such as algae species, location, season and extraction technique and can therefore vary tremendously between extracts. Due to the heterogeneity of fucoidan and the largely unknown relation between chemical structure and function, it remains challenging to use fucoidan in a medical context. The presented dissertation investigates the biological activity of different fucoidan extracts on molecular processes relevant for bone tissue regeneration and relates chemical properties of the extracts to the observed bioactivities. Mono- and co-cultures of human outgrowth endothelial cells (OEC) isolated from peripheral blood and human mesenchymal stem cells isolated from cancellous bone and differentiated towards an osteoblast lineage (MSC) were used as cell model systems to mimic the bone tissue environment. To come one step closer to the utilization of fucoidan as a therapeutic agent, a thermosensitive chitosan-collagen hydrogel-based delivery system with incorporated fucoidan was developed. 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00834-9$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00003285 5051 570 5550 Angiogenesis 5550 Bone 5550 Brown algae 5550 Endothelial cells 5550 Fucoidan 5550 Hydrogel 5550 MSC 5550 Osteogenesis