000K  utf8
1100  $c2023
1500  eng
2050  urn:nbn:de:gbv:8:3-2023-00304-3
2051  10.3390/pathogens12040591
3000  Ogun, Oluwamayowa Joshua
3010  Becker, Doreen
3010  Soremekun, Opeyemi S
3010  Thaller, Georg
4000  An In Silico Functional Analysis of Non-Synonymous Single-Nucleotide Polymorphisms of Bovine CMAH Gene and Potential Implication in Pathogenesis  [Ogun, Oluwamayowa Joshua]
4209  The sugar molecule N-glycolylneuraminic acid (Neu5Gc) is one of the most common sialic acids discovered in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) catalyses the conversion of N-acetylneuraminic acid (Neu5Ac) to Neu5Gc, and it is encoded by the CMAH gene. On the one hand, food metabolic incorporation of Neu5Gc has been linked to specific human diseases. On the other hand, Neu5Gc has been shown to be highly preferred by some pathogens linked to certain bovine diseases. We used various computational techniques to perform an in silico functional analysis of five non-synonymous single-nucleotide polymorphisms (nsSNPs) of the bovine CMAH (bCMAH) gene identified from the 1000 Bull Genomes sequence data. The c.1271C>T (P424L) nsSNP was predicted to be pathogenic based on the consensus result from different computational tools. The nsSNP was also predicted to be critical based on sequence conservation, stability, and post-translational modification site analysis. According to the molecular dynamic simulation and stability analysis, all variations promoted stability of the bCMAH protein, but mutation A210S significantly promoted CMAH stability. In conclusion, c.1271C>T (P424L) is expected to be the most harmful nsSNP among the five detected nsSNPs based on the overall studies. This research could pave the way for more research associating pathogenic nsSNPs in the bCMAH gene with diseases.
4950  https://doi.org/10.3390/pathogens12040591$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2023-00304-3$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00003623
5051  333.7
5051  610
5550  Bacteria
5550  Cmah
5550  Neu5ac
5550  Neu5gc
5550  Pathogenesis
5550  Sialic acid
5550  SNPs
5550  Virus