@Article{macau_mods_00003625, author = {D{\"o}rschmann, Philipp and Thalenhorst, Tabea and Seeba, Charlotte and Tischh{\"o}fer, Marie-Theres and Neupane, Sandesh and Roider, Johann Baptist and Alban, Susanne and Klettner, Alexa Karina}, title = {Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium}, journal = {International Journal of Molecular Sciences}, year = {2023}, publisher = {MDPI AG}, address = {Basel}, volume = {24}, number = {9}, keywords = {sulfated fucan; retinal pigment epithelium-specific 65 kDa protein (RPE65); vascular endothelial growth factor (VEGF); phagocytosis; gene expression; protectin (CD59); trans-epithelial electrical resistance (TEER); interleukin; toll-like receptor; polyinosinic:polycytidylic acid (PIC)}, abstract = {Fucoidans from brown algae are described as anti-inflammatory, antioxidative, and antiangiogenic. We tested two Saccharina latissima fucoidans (SL-FRO and SL-NOR) regarding their potential biological effects against age-related macular degeneration (AMD). Primary porcine retinal pigment epithelium (RPE), human RPE cell line ARPE-19, and human uveal melanoma cell line OMM-1 were used. Cell survival was assessed in tetrazolium assay (MTT). Oxidative stress assays were induced with erastin or H2O2. Supernatants were harvested to assess secreted vascular endothelial growth factor A (VEGF-A) in ELISA. Barrier function was assessed by measurement of trans-epithelial electrical resistance (TEER). Protectin (CD59) and retinal pigment epithelium-specific 65 kDa protein (RPE65) were evaluated in western blot. Polymorphonuclear elastase and complement inhibition assays were performed. Phagocytosis of photoreceptor outer segments was tested in a fluorescence assay. Secretion and expression of proinflammatory cytokines were assessed with ELISA and real-time PCR. Fucoidans were chemically analyzed. Neither toxic nor antioxidative effects were detected in ARPE-19 or OMM-1. Interleukin 8 gene expression was slightly reduced by SL-NOR but induced by SL-FRO in RPE. VEGF secretion was reduced in ARPE-19 by SL-FRO and in RPE by both fucoidans. Polyinosinic:polycytidylic acid induced interleukin 6 and interleukin 8 secretion was reduced by both fucoidans in RPE. CD59 expression was positively influenced by fucoidans, and they exhibited a complement and elastase inhibitory effect in cell-free assay. RPE65 expression was reduced by SL-NOR in RPE. Barrier function of RPE was transiently reduced.Phagocytosis ability was slightly reduced by both fucoidans in primary RPE but not in ARPE-19.}, issn = {1661-6596}, doi = {10.3390/ijms24097939}, url = {https://macau.uni-kiel.de/receive/macau_mods_00003625}, url = {https://doi.org/10.3390/ijms24097939}, file = {:https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00004837/ijms-24-07939.pdf:PDF}, language = {en} }