@Article{macau_mods_00003625,
  author = 	{D{\"o}rschmann, Philipp
		and Thalenhorst, Tabea
		and Seeba, Charlotte
		and Tischh{\"o}fer, Marie-Theres
		and Neupane, Sandesh
		and Roider, Johann Baptist
		and Alban, Susanne
		and Klettner, Alexa Karina},
  title = 	{Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium},
  journal = 	{International Journal of Molecular Sciences},
  year = 	{2023},
  publisher = 	{MDPI AG},
  address = 	{Basel},
  volume = 	{24},
  number = 	{9},
  keywords = 	{sulfated fucan; retinal pigment epithelium-specific 65 kDa protein (RPE65); vascular endothelial growth factor (VEGF); phagocytosis; gene expression; protectin (CD59); trans-epithelial electrical resistance (TEER); interleukin; toll-like receptor; polyinosinic:polycytidylic acid (PIC)},
  abstract = 	{Fucoidans from brown algae are described as anti-inflammatory, antioxidative, and antiangiogenic. We tested two Saccharina latissima fucoidans (SL-FRO and SL-NOR) regarding their potential biological effects against age-related macular degeneration (AMD). Primary porcine retinal pigment epithelium (RPE), human RPE cell line ARPE-19, and human uveal melanoma cell line OMM-1 were used. Cell survival was assessed in tetrazolium assay (MTT). Oxidative stress assays were induced with erastin or H2O2. Supernatants were harvested to assess secreted vascular endothelial growth factor A (VEGF-A) in ELISA. Barrier function was assessed by measurement of trans-epithelial electrical resistance (TEER). Protectin (CD59) and retinal pigment epithelium-specific 65 kDa protein (RPE65) were evaluated in western blot. Polymorphonuclear elastase and complement inhibition assays were performed. Phagocytosis of photoreceptor outer segments was tested in a fluorescence assay. Secretion and expression of proinflammatory cytokines were assessed with ELISA and real-time PCR. Fucoidans were chemically analyzed. Neither toxic nor antioxidative effects were detected in ARPE-19 or OMM-1. Interleukin 8 gene expression was slightly reduced by SL-NOR but induced by SL-FRO in RPE. VEGF secretion was reduced in ARPE-19 by SL-FRO and in RPE by both fucoidans. Polyinosinic:polycytidylic acid induced interleukin 6 and interleukin 8 secretion was reduced by both fucoidans in RPE. CD59 expression was positively influenced by fucoidans, and they exhibited a complement and elastase inhibitory effect in cell-free assay. RPE65 expression was reduced by SL-NOR in RPE. Barrier function of RPE was transiently reduced.Phagocytosis ability was slightly reduced by both fucoidans in primary RPE but not in ARPE-19.},
  issn = 	{1661-6596},
  doi = 	{10.3390/ijms24097939},
  url = 	{https://macau.uni-kiel.de/receive/macau_mods_00003625},
  url = 	{https://doi.org/10.3390/ijms24097939},
  file = 	{:https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00004837/ijms-24-07939.pdf:PDF},
  language = 	{en}
}