000K utf8 1100 $c2023 1500 eng 2050 urn:nbn:de:gbv:8:3-2023-00306-4 2051 10.3390/ijms24097939 3000 Dörschmann, Philipp 3010 Alban, Susanne 3010 Klettner, Alexa Karina 3010 Neupane, Sandesh 3010 Roider, Johann Baptist 3010 Seeba, Charlotte 3010 Thalenhorst, Tabea 3010 Tischhöfer, Marie-Theres 4000 Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium [Dörschmann, Philipp] 4209 Fucoidans from brown algae are described as anti-inflammatory, antioxidative, and antiangiogenic. We tested two Saccharina latissima fucoidans (SL-FRO and SL-NOR) regarding their potential biological effects against age-related macular degeneration (AMD). Primary porcine retinal pigment epithelium (RPE), human RPE cell line ARPE-19, and human uveal melanoma cell line OMM-1 were used. Cell survival was assessed in tetrazolium assay (MTT). Oxidative stress assays were induced with erastin or H2O2. Supernatants were harvested to assess secreted vascular endothelial growth factor A (VEGF-A) in ELISA. Barrier function was assessed by measurement of trans-epithelial electrical resistance (TEER). Protectin (CD59) and retinal pigment epithelium-specific 65 kDa protein (RPE65) were evaluated in western blot. Polymorphonuclear elastase and complement inhibition assays were performed. Phagocytosis of photoreceptor outer segments was tested in a fluorescence assay. Secretion and expression of proinflammatory cytokines were assessed with ELISA and real-time PCR. Fucoidans were chemically analyzed. Neither toxic nor antioxidative effects were detected in ARPE-19 or OMM-1. Interleukin 8 gene expression was slightly reduced by SL-NOR but induced by SL-FRO in RPE. VEGF secretion was reduced in ARPE-19 by SL-FRO and in RPE by both fucoidans. Polyinosinic:polycytidylic acid induced interleukin 6 and interleukin 8 secretion was reduced by both fucoidans in RPE. CD59 expression was positively influenced by fucoidans, and they exhibited a complement and elastase inhibitory effect in cell-free assay. RPE65 expression was reduced by SL-NOR in RPE. Barrier function of RPE was transiently reduced.Phagocytosis ability was slightly reduced by both fucoidans in primary RPE but not in ARPE-19. 4950 https://doi.org/10.3390/ijms24097939$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2023-00306-4$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00003625 5051 610 5550 gene expression 5550 interleukin 5550 phagocytosis 5550 polyinosinic:polycytidylic acid (PIC) 5550 protectin (CD59) 5550 retinal pigment epithelium-specific 65 kDa protein (RPE65) 5550 sulfated fucan 5550 toll-like receptor 5550 trans-epithelial electrical resistance (TEER) 5550 vascular endothelial growth factor (VEGF)