000K  utf8
1100  $c2023
1500  eng
2050  urn:nbn:de:gbv:8:3-2023-00306-4
2051  10.3390/ijms24097939
3000  Dörschmann, Philipp
3010  Alban, Susanne
3010  Klettner, Alexa Karina
3010  Neupane, Sandesh
3010  Roider, Johann Baptist
3010  Seeba, Charlotte
3010  Thalenhorst, Tabea
3010  Tischhöfer, Marie-Theres
4000  Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium  [Dörschmann, Philipp]
4209  Fucoidans from brown algae are described as anti-inflammatory, antioxidative, and antiangiogenic. We tested two Saccharina latissima fucoidans (SL-FRO and SL-NOR) regarding their potential biological effects against age-related macular degeneration (AMD). Primary porcine retinal pigment epithelium (RPE), human RPE cell line ARPE-19, and human uveal melanoma cell line OMM-1 were used. Cell survival was assessed in tetrazolium assay (MTT). Oxidative stress assays were induced with erastin or H2O2. Supernatants were harvested to assess secreted vascular endothelial growth factor A (VEGF-A) in ELISA. Barrier function was assessed by measurement of trans-epithelial electrical resistance (TEER). Protectin (CD59) and retinal pigment epithelium-specific 65 kDa protein (RPE65) were evaluated in western blot. Polymorphonuclear elastase and complement inhibition assays were performed. Phagocytosis of photoreceptor outer segments was tested in a fluorescence assay. Secretion and expression of proinflammatory cytokines were assessed with ELISA and real-time PCR. Fucoidans were chemically analyzed. Neither toxic nor antioxidative effects were detected in ARPE-19 or OMM-1. Interleukin 8 gene expression was slightly reduced by SL-NOR but induced by SL-FRO in RPE. VEGF secretion was reduced in ARPE-19 by SL-FRO and in RPE by both fucoidans. Polyinosinic:polycytidylic acid induced interleukin 6 and interleukin 8 secretion was reduced by both fucoidans in RPE. CD59 expression was positively influenced by fucoidans, and they exhibited a complement and elastase inhibitory effect in cell-free assay. RPE65 expression was reduced by SL-NOR in RPE. Barrier function of RPE was transiently reduced.Phagocytosis ability was slightly reduced by both fucoidans in primary RPE but not in ARPE-19.
4950  https://doi.org/10.3390/ijms24097939$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2023-00306-4$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00003625
5051  610
5550  gene expression
5550  interleukin
5550  phagocytosis
5550  polyinosinic:polycytidylic acid (PIC)
5550  protectin (CD59)
5550  retinal pigment epithelium-specific 65 kDa protein (RPE65)
5550  sulfated fucan
5550  toll-like receptor
5550  trans-epithelial electrical resistance (TEER)
5550  vascular endothelial growth factor (VEGF)