000K utf8 1100 $c2023 1500 eng 2050 urn:nbn:de:gbv:8:3-2023-00378-0 2051 10.1111/exd.14727 3000 Ochlich, Désirée 3010 Drerup, Katharina Antonia 3010 Gläser, Regine 3010 Harder, Jürgen 3010 Rademacher, Franziska 4000 The influence of the commensal skin bacterium Staphylococcus epidermidis on the epidermal barrier and inflammation$dImplications for atopic dermatitis [Ochlich, Désirée] 4209 The skin microbiota is a crucial component in maintaining cutaneous barrier function. Staphylococcus epidermidis is considered as a beneficial commensal member of the cutaneous microbiota promoting skin health. However, S. epidermidis is also frequently detectable in the skin of patients with the inflammatory skin disease atopic dermatitis (AD) and some studies reported a significantly higher presence of S. epidermidis in severe AD as compared to mild AD. Therefore, this study aimed to analyse the impact of S. epidermidis on the expression of cutaneous inflammatory mediators and skin barrier molecules. Various S. epidermidis skin-derived isolates activated the proinflammatory transcription factor NF-kappaB and induced expression of AD-associated proinflammatory cytokines in human primary keratinocytes and 3D skin equivalents. Skin barrier molecules such as filaggrin were downregulated by S. epidermidis. In general, AD-derived S. epidermidis strains elicited a higher response than strains derived from the skin of healthy individuals. Taken together, our results provide further evidence that the abundance of S. epidermidis in AD may trigger the inflammatory scenario associated with this disease. 4950 https://doi.org/10.1111/exd.14727$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2023-00378-0$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00003699 5051 610 5550 Atopic Dermtitis 5550 Dermatitis, Atopic 5550 Humans 5550 Inflammation 5550 Keratinocytes 5550 Keratinocytes 5550 Skin 5550 Skin Inflammation 5550 Staphylococcus epidermidis 5550 Staphylococcus epidermidis