PT Journal
AU Titov, I
   Rutschke, N
   Kraft, FA
   Köpke, M
   Nebling, E
   Gerken, M
TI Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices
SO Biomedical optics express
PY 2022
BP 6300
EP 6316
VL 13
IS 12
PU Optica Publishing Group
DI 10.1364/BOE.475358
WP https://macau.uni-kiel.de/receive/macau_mods_00003943
LA en
DE single-stranded DNA (ssDNA); fluorescence; optoelectronic; Förster resonance transfer (FRET); multiplex biomarker; measurements
SN 2156-7085
AB We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode (OLED) and an organic photodetector (OPD) with 0.5 mm by 0.5 mm device sizes has the potential to enable compact and low-cost fluorescence point-of-care (POC) devices for decentral multiplex biomedical testing. Here, we used two 6-FAM and BHQ1 labeled complementary ssDNA strands to form the Förster resonance transfer (FRET) upon the hybridization of the DNA. In this work we successfully show ssDNA hybridization sensing with samples diluted in TE buffer and investigate the detection of covalently bound 6-FAM-ssDNA on a glass surface for multiplex biomarker measurements.
PI Washington, DC
ER