000K  utf8
1100  $c2022
1500  eng
2050  urn:nbn:de:gbv:8:3-2023-00620-8
2051  10.1364/BOE.475358
3000  Titov, Igor
3010  Gerken, Martina
3010  Köpke, Markus
3010  Kraft, Fabio Aldo
3010  Nebling, Eric
3010  Rutschke, Nils
4000  Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices  [Titov, Igor]
4209  We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode (OLED) and an organic photodetector (OPD) with 0.5 mm by 0.5 mm device sizes has the potential to enable compact and low-cost fluorescence point-of-care (POC) devices for decentral multiplex biomedical testing. Here, we used two 6-FAM and BHQ1 labeled complementary ssDNA strands to form the Förster resonance transfer (FRET) upon the hybridization of the DNA. In this work we successfully show ssDNA hybridization sensing with samples diluted in TE buffer and investigate the detection of covalently bound 6-FAM-ssDNA on a glass surface for multiplex biomarker measurements.
4950  https://doi.org/10.1364/BOE.475358$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2023-00620-8$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00003943
5051  600
5550  fluorescence
5550  Förster resonance transfer (FRET)
5550  measurements
5550  multiplex biomarker
5550  optoelectronic
5550  single-stranded DNA (ssDNA)