000K utf8 1100 $c2023 1500 eng 2050 urn:nbn:de:gbv:8:3-2024-00034-8 2051 10.3390/md21060339 3000 Kirsten, Nora 3010 Blümel, Martina 3010 Fuchs, Sabine 3010 Meyer, Anne S 3010 Mikkelsen, Maria Dalgaard 3010 Nguyen, Thuan Thi 3010 Ohmes, Julia 3010 Seekamp, Andreas 3010 Tasdemir, Deniz 3010 Wang, Fanlu 4000 Impact of Enzymatically Extracted High Molecular Weight Fucoidan on Lipopolysaccharide-Induced Endothelial Activation and Leukocyte Adhesion$hMDPI AG [Kirsten, Nora] 4030 $nMDPI AG 4209 The endothelial cell lining creates an interface between circulating blood and adjoining tissue and forms one of the most critical barriers and targets for therapeutical intervention. Recent studies suggest that fucoidans, sulfated and fucose-rich polysaccharides from brown seaweed, show multiple promising biological effects, including anti-inflammatory properties. However, their biological activity is determined by chemical characteristics such as molecular weight, sulfation degree, and molecular structure, which vary depending on the source, species, and harvesting and isolation method. In this study, we investigated the impact of high molecular weight (HMW) fucoidan extract on endothelial cell activation and interaction with primary monocytes (MNCs) in lipopolysaccharide (LPS)-induced inflammation. Gentle enzyme-assisted extraction combined with fractionation by ion exchange chromatography resulted in well-defined and pure fucoidan fractions. FE_F3, with a molecular weight ranging from 110 to 800 kDa and a sulfate content of 39%, was chosen for further investigation of its anti-inflammatory potential. We observed that along with higher purity of fucoidan fractions, the inflammatory response in endothelial mono- and co-cultures with MNCs was reduced in a dose-dependent manner when testing two different concentrations. This was demonstrated by a decrease in IL-6 and ICAM-1 on gene and protein levels and a reduced gene expression of TLR-4, GSK3β and NF-kB. Expression of selectins and, consequently, the adhesion of monocytes to the endothelial monolayer was reduced after fucoidan treatment. These data indicate that the anti-inflammatory effect of fucoidans increases with their purity and suggest that fucoidans might be useful in limiting the inflammatory response of endothelial cells in cases of LPS-induced bacterial infection. 4950 https://doi.org/10.3390/md21060339$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2024-00034-8$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00004315 5051 500 5051 610 5550 Anti-Inflammatory Agents 5550 Endothelial Cells 5550 Fucoidan 5550 Fucus Evanescens 5550 Hmwf 5550 Inflammation 5550 Leukocytes 5550 Lipopolysaccharide 5550 Lipopolysaccharides 5550 Molecular Weight 5550 Monocytes 5550 Outgrowth Endothelial Cells 5550 Polysaccharides