000K  utf8
1100  $c2024
1500  eng
2050  urn:nbn:de:gbv:8:3-2025-00368-4
2051  10.3390/jof10070487
3000  Kollath-Leiß, Krisztina
3010  Freund, Anna Sophia
3010  Kempken, Frank
3010  Repnik, Urska
3010  Winkelmann, Heinrich
3010  Winter, Hannes
4000  $hMDPI AG  [Kollath-Leiß, Krisztina]
4000  The First Observation of the Filamentous Fungus Neurospora crassa Growing in the Roots of the Grass Brachypodium distachyon$hMDPI AG  [Kollath-Leiß, Krisztina]
4030  $nMDPI AG
4209  The model organism Neurospora crassa has been cultivated in laboratories since the 1920s and its saprotrophic lifestyle has been established for decades. However, beyond their role as saprotrophs, fungi engage in intricate relationships with plants, showcasing diverse connections ranging from mutualistic to pathogenic. Although N. crassa has been extensively investigated under laboratory conditions, its ecological characteristics remain largely unknown. In contrast, Brachypodium distachyon, a sweet grass closely related to significant crops, demonstrates remarkable ecological flexibility and participates in a variety of fungal interactions, encompassing both mutualistic and harmful associations. Through a comprehensive microscopic analysis using electron, fluorescence, and confocal laser scanning microscopy, we discovered a novel endophytic interaction between N. crassa and B. distachyon roots, where fungal hyphae not only thrive in the apoplastic space and vascular bundle but also may colonize plant root cells. This new and so far hidden trait of one of the most important fungal model organisms greatly enhances our view of N. crassa, opening new perspectives concerning the fungus' ecological role. In addition, we present a new tool for studying plant-fungus interspecies communication, combining two well-established model systems, which improves our possibilities of experimental design on the molecular level.
4950  https://doi.org/10.3390/jof10070487$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2025-00368-4$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00005899
5051  580
5550  Brachypodium Distachyon
5550  Endophytic Lifestyle
5550  neurospora crassa
5550  Plant–fungus Interaction