PT Journal
AU Eichhorn, A
   Neumann, F
   Bäumler, C
   Gutsmann, I
   Grobe, O
   Schlüter, F
   Müller, S
   Krumbholz, A
TI Assessment of the Diagnostic Performance of Fully Automated Hepatitis E Virus (HEV) Antibody Tests
SO Diagnostics (Basel, Switzerland)
JI Diagnostics
PY 2024
BP 602
VL 14
IS 6
PU MDPI AG
DI 10.3390/diagnostics14060602
WP https://macau.uni-kiel.de/receive/macau_mods_00005902
LA en
DE Hepatitis E virus; Immunoassays; Percentage Agreement; Polyclonal Stimulation
SN 2075-4418
AB The detection of anti-hepatitis E virus (HEV) antibodies contributes to the diagnosis of hepatitis E. The diagnostic suitability of two automated chemiluminescence immunoassays (CLIAs, LIAISON® MUREX Anti-HEV IgG/Anti-HEV IgM test, DiaSorin) was assessed by comparison with the results of a combination of enzyme immunoassays and immunoblots (recomWell HEV IgG/IgM ELISA, recomLine HEV IgG/IgM, MIKROGEN). Samples with a deviating result were analyzed with the WANTAI ELISAs. Compared to the recomWell ELISAs, the Anti-HEV IgG CLIA had a percentage overall agreement (POA) of 100% (149/149; 95% CI: 97.5-100%) and the Anti-HEV IgM CLIA had a POA of 83.3% (85/102; 95% CI: 74.9-89.3%); considering the recomLine HEV IgM results, the POA was 71.7% (38/53; 95% CI: 58.4-82%). The WANTAI test confirmed 52.9% (9/17) of negative CLIA IgMs; HEV RNA was not detectable. Since acute infection with the Epstein-Barr virus (EBV) or human cytomegalovirus (CMV) may influence the results of other serological assays, HEV antibodies were examined in 17 EBV and 2 CMV patients: One had an isolated and probably unspecific HEV IgM in the CLIA, as HEV RNA was not detectable. Both CLIAs are well suited for HEV diagnostics, but isolated IgM should be confirmed. An acute EBV/CMV infection can influence HEV serodiagnostics.
PI Basel
ER