000K  utf8
1100  $c2024
1500  eng
2050  urn:nbn:de:gbv:8:3-2025-00371-2
2051  10.3390/diagnostics14060602
3000  Eichhorn, Anna
3010  Bäumler, Carina
3010  Grobe, Olaf
3010  Gutsmann, Imke
3010  Krumbholz, Andi
3010  Müller, Sina
3010  Neumann, Franziska
3010  Schlüter, Frieda
4000  Assessment of the Diagnostic Performance of Fully Automated Hepatitis E Virus (HEV) Antibody Tests$hMDPI AG  [Eichhorn, Anna]
4030  $nMDPI AG
4209  The detection of anti-hepatitis E virus (HEV) antibodies contributes to the diagnosis of hepatitis E. The diagnostic suitability of two automated chemiluminescence immunoassays (CLIAs, LIAISON® MUREX Anti-HEV IgG/Anti-HEV IgM test, DiaSorin) was assessed by comparison with the results of a combination of enzyme immunoassays and immunoblots (recomWell HEV IgG/IgM ELISA, recomLine HEV IgG/IgM, MIKROGEN). Samples with a deviating result were analyzed with the WANTAI ELISAs. Compared to the recomWell ELISAs, the Anti-HEV IgG CLIA had a percentage overall agreement (POA) of 100% (149/149; 95% CI: 97.5-100%) and the Anti-HEV IgM CLIA had a POA of 83.3% (85/102; 95% CI: 74.9-89.3%); considering the recomLine HEV IgM results, the POA was 71.7% (38/53; 95% CI: 58.4-82%). The WANTAI test confirmed 52.9% (9/17) of negative CLIA IgMs; HEV RNA was not detectable. Since acute infection with the Epstein-Barr virus (EBV) or human cytomegalovirus (CMV) may influence the results of other serological assays, HEV antibodies were examined in 17 EBV and 2 CMV patients: One had an isolated and probably unspecific HEV IgM in the CLIA, as HEV RNA was not detectable. Both CLIAs are well suited for HEV diagnostics, but isolated IgM should be confirmed. An acute EBV/CMV infection can influence HEV serodiagnostics.
4950  https://doi.org/10.3390/diagnostics14060602$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2025-00371-2$xR$3Volltext$534
4961  https://macau.uni-kiel.de/receive/macau_mods_00005902
5051  610
5550  Hepatitis E virus
5550  Immunoassays
5550  Percentage Agreement
5550  Polyclonal Stimulation