000K utf8 1100 $c2018 1500 eng 2050 urn:nbn:de:gbv:8:3-2025-00395-0 2051 10.3389/fmicb.2018.00728 3000 Domin, Hanna 3010 Buttlar, Jann 3010 Fraune, Sebastian 3010 Hentschel Humeida, Ute 3010 Scotti, Marco 3010 Zurita-Gutiérrez, Yazmín H 4000 $hFrontiers Media SA [Domin, Hanna] 4000 Predicted Bacterial Interactions Affect in Vivo Microbial Colonization Dynamics in Nematostella$hFrontiers Media SA [Domin, Hanna] 4030 $nFrontiers Media SA 4209 The maintenance and resilience of host-associated microbiota during development is a fundamental process influencing the fitness of many organisms. Several host properties were identified as influencing factors on bacterial colonization, including the innate immune system, mucus composition, and diet. In contrast, the importance of bacteria-bacteria interactions on host colonization is less understood. Here, we use bacterial abundance data of the marine model organism Nematostella vectensis to reconstruct potential bacteria-bacteria interactions through co-occurrence networks. The analysis indicates that bacteria-bacteria interactions are dynamic during host colonization and change according to the host's developmental stage. To assess the predictive power of inferred interactions, we tested bacterial isolates with predicted cooperative or competitive behavior for their ability to influence bacterial recolonization dynamics. Within 3 days of recolonization, all tested bacterial isolates affected bacterial community structure, while only competitive bacteria increased bacterial diversity. Only 1 week after recolonization, almost no differences in bacterial community structure could be observed between control and treatments. These results show that predicted competitive bacteria can influence community structure for a short period of time, verifying the in silico predictions. However, within 1 week, the effects of the bacterial isolates are neutralized, indicating a high degree of resilience of the bacterial community. 4950 https://doi.org/10.3389/fmicb.2018.00728$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2025-00395-0$xR$3Volltext$534 4961 https://macau.uni-kiel.de/receive/macau_mods_00005927 5051 590 5550 Bacteria–bacteria Interactions 5550 Cnidaria 5550 Community ecology 5550 Correlation Networks 5550 Holobiont 5550 Host–microbe Interactions 5550 Metaorganism 5550 Resilience