Generation and Maintenance of T Follicular Helper Cells

A transcriptome analysis of Tfh and Non-Tfh cells revealed 19 signature genes with unknown function in Tfh cells. Retroviral infection of transgenic T cells allowed effective screening for these gene’s function by genetic overexpression or CRISPR Cas9-mediated knock out. Adoptive transfer of modified cells enabled the tracking of antigen-specific, modified T cells in vivo.

The in Tfh cells significantly downregulated Rara gene positively influenced the humoral immune response upon overexpression, while targeting the remaining 18 genes did not affect Tfh cell biology.


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