Successful PCR-based dermatophyte identification at species level in PAS-positive formalin-fixed skin biopsies and nail samples

In our pilot study, we confirmed that subsequent PCR analysis of PAS-positive skin samples is a useful methodological improvement in dermatophyte diagnostics. Especially in the case of nail material from histological specimens, subsequent PCR detection can be beneficial. The new S1 guideline on onychomycosis states that histological examination of the nail can be helpful, especially in previously treated patients with negative mycological findings but with strong suspicion of onychomycosis. Our study now shows for the first time that dermatophyte
species can be identified in formalin-fixed PAS-positive nail samples using a commercially available PCR assay. With our report, we would like to encourage the testing and further development of this PCR detection method in other laboratories. The use of a standardized commercialassay should facilitate compliance with the requirements of the European Parliament Regulation 2017/746 on in vitro diagnostics.

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